The purpose of preparing this guideline is to provide comprehensive, evidence-based and culturally-sensitive medico-legal services related to gender affirmative care of transpersons and to utilize it as an advocacy tool for good practices in this region. The objective of compiling this good practice guide is to facilitate health professionals engaged in providing gender transition related services to transgender persons. We hope that this evidence-based, culturally sensitive guide prepared with the help of medical and legal experts experienced in this field, would contribute in developing much needed multi-disciplinary collaborative gender affirmative care in this region. These guidelines can be used by health professionals, by individuals, their families, and social institutions to understand how they can assist with promoting optimal health for members of this diverse population. Our attempt has been to use non-technical language as much as possible while compiling this guideline so that any interested individual including legal experts, transgender persons, and activists engaged in this field can obtain an overview of medico-legal aspects of gender affirmative therapy.